the 50–100 μl permeabilization wash buffer was added Search Results


pcr buffer  (Thermo Fisher)
99
Thermo Fisher pcr buffer
Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr buffer - by Bioz Stars, 2026-09
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pc-3 cell suspension  (Corning Life Sciences)
90
Corning Life Sciences pc-3 cell suspension
Pc 3 Cell Suspension, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc-3 cell suspension - by Bioz Stars, 2026-09
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50% matrigel  (Corning Life Sciences)
90
Corning Life Sciences 50% matrigel
50% Matrigel, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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50% matrigel - by Bioz Stars, 2026-09
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rna lysis buffer  (Zymo Research)
96
Zymo Research rna lysis buffer
Rna Lysis Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/RNA+Lysis+Buffer/pmc05137741-468-8-17
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rna lysis buffer - by Bioz Stars, 2026-09
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50 ng/μl matrigel solution  (Becton Dickinson)
90
Becton Dickinson 50 ng/μl matrigel solution
50 Ng/μl Matrigel Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
50 ng/μl matrigel solution - by Bioz Stars, 2026-09
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tmb membrane peroxidase substrate  (Funakoshi ltd)
90
Funakoshi ltd tmb membrane peroxidase substrate
Tmb Membrane Peroxidase Substrate, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
tmb membrane peroxidase substrate - by Bioz Stars, 2026-09
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annexin v binding buffer  (Becton Dickinson)
90
Becton Dickinson annexin v binding buffer
FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with <t>Annexin</t> <t>V</t> and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.
Annexin V Binding Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/annexin+v+binding+buffer/pmc08350342-83-23-27
Average 90 stars, based on 1 article reviews
annexin v binding buffer - by Bioz Stars, 2026-09
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rnase inhibitor  (Promega)
90
Promega rnase inhibitor
FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with <t>Annexin</t> <t>V</t> and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.
Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/rnasin/pmc09531950-411-8-33
Average 90 stars, based on 1 article reviews
rnase inhibitor - by Bioz Stars, 2026-09
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poc capture probe (0.75 pmole/ml in coupling buffer: 500 mm na2hpo4 ph 8.5, 0.5 mm edta)  (Corning Life Sciences)
90
Corning Life Sciences poc capture probe (0.75 pmole/ml in coupling buffer: 500 mm na2hpo4 ph 8.5, 0.5 mm edta)
FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with <t>Annexin</t> <t>V</t> and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.
Poc Capture Probe (0.75 Pmole/Ml In Coupling Buffer: 500 Mm Na2hpo4 Ph 8.5, 0.5 Mm Edta), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/phosphate+buffered+saline/us08497250-290-9-37
Average 90 stars, based on 1 article reviews
poc capture probe (0.75 pmole/ml in coupling buffer: 500 mm na2hpo4 ph 8.5, 0.5 mm edta) - by Bioz Stars, 2026-09
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ice cold lysis buffer  (Thermo Fisher)
99
Thermo Fisher ice cold lysis buffer
FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with <t>Annexin</t> <t>V</t> and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.
Ice Cold Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/HEPES/pm35303367-319-26-49
Average 99 stars, based on 1 article reviews
ice cold lysis buffer - by Bioz Stars, 2026-09
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ripa buffer  (Thermo Fisher)
99
Thermo Fisher ripa buffer
FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with <t>Annexin</t> <t>V</t> and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.
Ripa Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+50%E2%80%93100+%CE%BCl+permeabilization+wash+buffer+was+added/RIPA+buffer/bio_rxiv__2025__07__16__665154-226-11-45
Average 99 stars, based on 1 article reviews
ripa buffer - by Bioz Stars, 2026-09
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Image Search Results


FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with Annexin V and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.

Journal: Frontiers in Oncology

Article Title: FOXM1-AKT Positive Regulation Loop Provides Venetoclax Resistance in AML

doi: 10.3389/fonc.2021.696532

Figure Lengend Snippet: FOXM1 knockdown in KG-1 cells increases their sensitivity to venetoclax. KG-1-Control and KG-1-FOXM1-KD cells were treated with indicated concentrations of venetoclax for 24 hours. (A) Total protein samples were purified immediately after treatment and analyzed via immunoblotting with indicated antibodies. Apoptotic activity was evaluated based on caspase-3 cleavage, β-actin was used as an internal loading control. (B) Cells were harvested immediately after treatment and stained with Annexin V and DAPI. Flow cytometry-based Annexin V assay was performed to identify viable (DAPI low /Annexin V low , black), early apoptotic (DAPI low /Annexin V high , green), early necrotic (DAPI high /Annexin V low , blue) and dead (DAPI high /Annexin V high , red) cells.

Article Snippet: After treatment the cells were harvested by centrifugation, washed twice with ice-cold PBS, and 500 000 cells were resuspended in 100 μL of Annexin V Binding Buffer (BD Biosciences, San Jose, CA, USA).

Techniques: Purification, Western Blot, Activity Assay, Staining, Flow Cytometry, Annexin V Assay